Bioorthogonal chemistry

Project information

  • Publication : Strain-Promoted Alkyne- Azide Cycloaddition (SPAAC) reaction of novel EpCAM aptamer- fluorescent conjugate for cancer cells imaging, N. Subramanian, Jagadeesh Babu Sreemanthula, BS Balaji, Jagat Kanwar, Jyotirmay Biswas and S. Krishnakumar, Chem. Commun., 2014, 11810-13.
    Appeared in the inside cover page.

For the first time, a novel EpCAM aptamer (SYL3C)-DIBO-AF594 fluorescent conjugate was synthesised by bioorthogonal chemistry utilizing a strain promoted alkyne‚Äďazide cycloaddition (copper free click) reaction (SPAAC). The obtained conjugate showed target specific binding and aided in the imaging of various EpCAM positive cancer cell lines like MCF-7, MDA-MB-453, Weri-RB1 and PC-3.

Collaborator: Dr. S. Krishnakumar, MD, Deputy Director Research, Head L&T Ocular Pathology Department, Vision Research Foundation, Sankara Nethralaya, Chennai, India.